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Journal: iScience
Article Title: Aerobicity stimulon in Escherichia coli revealed using multi-scale computational systems biology of respiratory variants
doi: 10.1016/j.isci.2026.114715
Figure Lengend Snippet: Impact of carbon source on ETS variants Metabolic flux routing of (A) glucose, (B) succinate, and (C) glycerol in E. coli central carbon metabolism. Growth rate of ETS variants on (D) succinate and (E) glycerol as carbon source before and after growth rate optimization by adaptive laboratory evolution. All laboratory evolutions were performed with three independently evolving lineages. The data from the final three flasks of the evolution experiments were used to show the growth rates of evolved strains. uXH: unevolved X protons per electron ETS variant, eXH: evolved X protons per electron ETS variant. The boxplot depicts the distribution of individual growth rate values for each strain under the specified growth condition. shows the post-ALE growth rate characterization of evolved strains to highlight the significant growth improvement. Acronyms- EMP: Embden-Meyerhof-Parnas Pathway, ED: Entner-Doudoroff Pathway, oxPPP: Oxidative Pentose Phosphate Pathway, TCA: Tricarboxylic Acid Cycle, g6p: D-Glucose-6-Phosphate, f6p: D-Fructose-6-Phosphate, 6 pg: 6-Phospho-D-Gluconate, g3p: Glyceraldehyde 3-Phosphate, pep: Phosphoenolpyruvate, pyr: Pyruvate, accoa: Acetyl-CoA, ru5p: Ribulose 5-Phosphate, r5p: Ribose 5-Phosphate, mal: Malate, glc: Glucose, succ: Succinate, glyc: Glycerol, WT: Wild Type, SMOS: Succinate Minimal Media Optimized WT Strain, GlyMOS: Glycerol Minimal Media Optimized WT Strain.
Article Snippet:
Techniques: Variant Assay